Ds Tagging Lines for Maize(转）
| 06月 28th, 2007 | by zhongtiannongmin |
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Dear MaizeCooperators,
As many
of you know, we have been developing a sequence-indexed collection
of Ds insertions
(http://www.plantgdb.org/prj/AcDsTagging/). Each line contains one or two novel Ds insertions that are
maintained in a uniform W22 inbred line. To date
we have generated sequence flanking approximately 900 Ds insertion
sites. We are actively
developing resources to make screening this collection user
friendly. These resources include several
periodically updated web pages:
of you know, we have been developing a sequence-indexed collection
of Ds insertions
(http://www.plantgdb.org/prj/AcDsTagging/). Each line contains one or two novel Ds insertions that are
maintained in a uniform W22 inbred line. To date
we have generated sequence flanking approximately 900 Ds insertion
sites. We are actively
developing resources to make screening this collection user
friendly. These resources include several
periodically updated web pages:
A BLAST page to query our
fDs collection with your favorite genes or BACs, etc. of
interest:
fDs collection with your favorite genes or BACs, etc. of
interest:
Ds insertion sites mapped to
BAC assemblies:
BAC assemblies:
Ds insertion sites mapped to
GSS assemblies:
GSS assemblies:
We have
also generated a browsable list of putative gene insertion sites
using the MAGI assemblies and their gene annotation as a
framework:
also generated a browsable list of putative gene insertion sites
using the MAGI assemblies and their gene annotation as a
framework:
and are
working on incorporating Alice Barkan’s POG annotations
(http://plantrbp.uoregon.edu/) to ascribe
putative functions to Ds flanking
sequences.
working on incorporating Alice Barkan’s POG annotations
(http://plantrbp.uoregon.edu/) to ascribe
putative functions to Ds flanking
sequences.
We invite
and encourage the maize research community to explore the
collection. Seed stocks for all lines are
currently available and can be ordered from the Brutnell lab
($40/line
academia; $100/line
industry). You will need to fill out an order
form (http://www.plantgdb.org/prj/AcDsTagging/order_instructions.php ) and
we will distribute approximately 10 seed to you. All Ds insertions
segregate 1:1 in our testcross populations, so you will need to
self pollinate all the plants to ensure recovery of the Ds insertion and to uncover
recessive mutations. When you order a line we
will also propagate the materials in either our summer or winter
nursery to ensure that the seed stocks are maintained and to
provide you with additional seedstocks (@100k) once we harvest the
bulked materials. None of this material is
transgenic and no MTA is required.
and encourage the maize research community to explore the
collection. Seed stocks for all lines are
currently available and can be ordered from the Brutnell lab
($40/line
academia; $100/line
industry). You will need to fill out an order
form (http://www.plantgdb.org/prj/AcDsTagging/order_instructions.php ) and
we will distribute approximately 10 seed to you. All Ds insertions
segregate 1:1 in our testcross populations, so you will need to
self pollinate all the plants to ensure recovery of the Ds insertion and to uncover
recessive mutations. When you order a line we
will also propagate the materials in either our summer or winter
nursery to ensure that the seed stocks are maintained and to
provide you with additional seedstocks (@100k) once we harvest the
bulked materials. None of this material is
transgenic and no MTA is required.
Please
keep in mind that this is a growing collection. Each month for the next 2 + years we will be placing approximately
200 new Ds insertions on the physical
map, so check back often!
keep in mind that this is a growing collection. Each month for the next 2 + years we will be placing approximately
200 new Ds insertions on the physical
map, so check back often!
Your feedback is greatly
appreciated.
Best
regards,
regards,
Tom
Brutnell, Boyce Thompson Institute, Cornell University
Brutnell, Boyce Thompson Institute, Cornell University
Erik
Vollbrecht, Iowa State University
Vollbrecht, Iowa State University
Volker
Brendel, Iowa State University
Brendel, Iowa State University
Thomas
Brutnell
Brutnell
Associate
Scientist
Scientist
Boyce Thompson
Institute
Institute
Cornell
University
University
Tower Road
Ithaca, NY 14853
(607) 254-8656